G-banding and Fluorescence In Situ Hybridization in Childhood Acute Myeloid Leukemia from South India

Bone marrow samples obtained from the patients were cultured in RPMI 1640 (Gibco, Invitrogen, USA) medium that contained 15% fetal bovine serum for 24 and 48 hours at 37 C. After incubation, the cells were exposed to colcemid (0.10 μg/mL, Gibco) for 30 minutes, followed by hypotonic treatment (0.075 M KCl) Abstract Background: ence of recurrent cytogenetic abnormalities. Diagnostic clonal chromosomal abnormalities provide important prognostic information and are among the most important factors in predicting initial response to chemotherapy, duration of remission and overall survival. Methods: emia (AML). Results: The culture success rate was 94%, clonal chromosomal abnormalities constituted 62% and recurrent chromosomal abnormalities were 56%. In the favorable prognostic category, there were 51.6% of cases with t(8;21)(q22;q22), 16.1% had t(15;17)(q22;q21), and a total